АНАЛИЗ АДАПТИВНОСТИ ЧЕЛОВЕКА К УСЛОВИЯМ НОВИЗНЫ ДЕЯТЕЛЬНОСТИ ПО ПОКАЗАТЕЛЯМ РЕЗУЛЬТАТИВНОСТИ И ВАРИАБЕЛЬНОСТИ КАРДИОРИТМА ПРИ СЕНСО-МОТОРНОМ ТЕСТИРОВАНИИ
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НИИ ноpмальной физиологии им. П.К. Анохина
Год издания: 2015
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that 50% of neurotransmitter quanta were released within 0.88±0.05 msec and 90% within 1.19±0.07 msec. Denervation (seven experiments, extracellular Ca2+ ion concentration 0.25-0.3 mM, the EPC quantal content was essentially identical to control experiments) lead to an increase in desynchronization of quantal release. The distribution of synaptic delays had a mean of 1.85±0.19 msec, a dispersion of 1.92±0.27; construction of cumulative curves demonstrated that 50% of neurotransmitter quanta were released within 1.06±0.03 msec and 90% within 3.52±0.47 msec. The topography of transmitter release in nerve endings was studied using a three-microelectrode method [5]. In control transmitter release did not occur throughout the whole surface of the nerve ending, but only in specific sites. The configurations and sizes of the transmitter release sites, as well as the distances between them, were identical to the positions and sizes of active zones described in ultrastructural studies [3, 5, 1]. Denervation caused groupings of release sites lost their outlines, became blurred, and transmitter release occurred over the whole surface of the nerve ending. But in some experiments increased probability of transmitter release in certain synaptic regions were observed that likely coincided with the location of large fragments of partially disorganized active zones. Thus, obtained data allowed to consider that denervation caused disorganization of active zones that led to decrease in synatic efficiency and increase in the time course of transmitter release. The results presented here are in agreement with previously obtained data on the studying of transmitter release in the frog neuromuscular junction after exposure to calcium-free solution that was another experimental approach led to disorganization of active zones probably due to impairment of the cytoskeleton [1]. The work was supported by RFBR (grant № 14-04-01232-а) and RSСF (grant № 14-15-00847). References. 1. Zefirov A.L., R. D. Mukhamedzianov,M. G. Minlebaev et al. // Ross Fiziol Zh Im I M Sechenova. 2002. V.88, №2. P. 191-204. 2. Ko C.P. // J. Physiol. 1981. Vol. 321. P. 627-639. 3. Pawson P.A., Grinnel A.D., Wolowske B. // 1998. J. Neurocytol. Vol. 27. P. 379-391. 4. Rizzoli S.O. // The EMBO Journal. 2014. Vol. 33, №8. P. 788 822. 5. Zefirov A.L., Benish T., Fatkullin N., et. al. // Nature. 1995. Vol, 376. P. 393-394. DOI:10.12737/12328 АНАЛИЗ АДАПТИВНОСТИ ЧЕЛОВЕКА К УСЛОВИЯМ НОВИЗНЫ ДЕЯТЕЛЬНОСТИ ПО ПОКАЗАТЕЛЯМ РЕЗУЛЬТАТИВНОСТИ И ВАРИАБЕЛЬНОСТИ КАРДИОРИТМА ПРИ СЕНСО-МОТОРНОМ ТЕСТИРОВАНИИ Гриднева Н.А., Муртазина Е.П., Журавлев Б.В. ФГБНУ НИИ нормальной физиологии им.П.К.Анохина, Москва, РФ n.gridneva@nphys.ru